. In this system, ligand is a molecule which can detect the antibody and is covalently coupled to an enzyme such as peroxidase. This binds the test antibody and after free ligand is washed away (6) the bound ligand is visualized by the addition of chromogen (7) – a colourless substrate which is acted on by the enzyme portion of the ligand to produce a coloured end-product. A developed plate is shown in the lower panel (8). The amount of test antibody is measured by assessing the amount of coloured end-product by optical density scanning of the plate.
· التاريخ: الأحد ديسمبر 16, 2007 · عدد مرات المشاهدة: 312 ·