Immunoelectrophoresis allows the comparison of complicated mixtures of antigen such as are found in serum. (1) Antigens are separated in an agar gel by placing an electric charge across it. The gel’s pH is chosen so that positively charged proteins move to the negative electrode and negatively charged proteins to the positive. (2) A trough is then cut between the wells and filled with the antibody, which is left to diffuse. (3) The antigens and antibody form precipitin arcs. The lower Figure shows the complex patterns which result when different sera are analyzed by electrophoresis.
· التاريخ: الأحد ديسمبر 16, 2007 · عدد مرات المشاهدة: 302 ·