Epi-Fluorescence Illumination - أطلس مختبرات العرب

Until very recently, fluorescence illumination was an option available only on research-level compound microscopes equipped with specialized high-numerical aperture objectives. The need for this technique in stereomicroscopy has escalated with the introduction of genetically encoded and biologically specific fluorescent proteins such as GFP (Green Fluorescent Protein).

The application of stereomicroscopes for GFP observation is now so prevalent that stereo fluorescence illuminators are more frequently referred to as GFP illuminators, even though they can be utilized for many other applications in both the life sciences and the electronics manufacturing industry. Large specimens, such as larvae, nematodes, Zebrafish, oocytes, and mature insects can be easily selected and manipulated when they are labeled with GFP and illuminated by fluorescence techniques. The fluorescence illumination reveals which organisms are producing the fluorescent protein and the stereoscopic vision coupled to a large field of view and ample working distance enables observers to conduct experiments with forceps, pipettes, or micromanipulators. Other, more conventional, specimens are also easily observed and recorded using stereomicroscopes with fluorescence illumination